AarI CACCTGCNNNNNNNNN 11
AatII GACGTC 5
AbsI CCTCGAGG 2 
AccI GTAGAC 2
AccI GTATAC 2
AccI GTCGAC 2
AccI GTCTAC 2
Acc65I GCTACC 1
AclI AACGTT 2
AcuI CTGAAGNNNNNNNNNNNNNNNNN 22
AfeI AGCGCT 3
AflII CTTAAG 1
AflIII ACACGT 1
AflIII ACATGT 1
AflIII ACGCGT 1
AflIII ACGTGT 1
AgeI ACCGGT 1
AhdI GACNNNNNGTC 6
AleI CACNNNNGTG 5
AlwNI CAGNNNCTG 6
ApaI GGGCCC 5
ApaLI GTGCAC 1
ApoI AAATTC 1
ApoI AAATTT 1
ApoI GAATTC 1
ApoI GAATTT 1
AscI GGCGCGCC 2
AseI ATTAAT 2
AsiSI GCGATCGC 5
AvaI CCCGAG 1
AvaI CCCGGG 1
AvaI CTCGAG 1
AvaI CTCGGG 1
AvrII CCTAGG 1
BaeGI GGGCAC 5
BaeGI GGGCCC 5
BaeGI GTGCAC 5
BaeGI GTGCCC 5
BamHI GGATCC 1
BanI GGCACC 1
BanI GGCGCC 1
BanI GGTACC 1
BanI GGTGCC 1
BanII GAGCCC 5
BanII GAGCTC 5
BanII GGGCCC 5
BanII GGGCTC 5
BbsI GAAGACNNNNNNN 8
BbvCI CCTCAGC 2
BciVI GTATCCNNNNNNN 12
BclI TGATCA 1
BfuAI ACCTGCNNNNNNNNN 10
BglI GCCNNNNNGGC 7
BglII AGATCT 1
BlpI GCTNAGC 2
Bme1580I GGGCAC 5
Bme1580I GGGCCC 5
Bme1580I GTGCAC 5
Bme1580I GTGCCC 5
BmeT110I CCCGAG 2
BmeT110I CCCGGG 2
BmeT110I CTCGAG 2
BmeT110I CTCGGG 2
BmgBI CACGTC 3
BmrI ACTGGGNNNNNN 11
BmtI GCTAGC 5
BpmI CTGGAGNNNNNNNNNNNNNNNNN 22
Bpu10I CCTNAGC 2
BpuEI CTTGAGNNNNNNNNNNNNNNNNN 22
BsaI GGTCTCNNNNNN 7
BsaAI CACGTA 3
BsaAI CACGTG 3
BsaAI TACGTA 3
BsaAI TACGTG 3
BsaBI GATNNNNATC 5
BsaHI GACGCC 2
BsaHI GACGTC 2
BsaHI GGCGCC 2
BsaHI GGCGTC 2
BsaWI ACCGGA 1
BsaWI ACCGGT 1
BsaWI TCCGGA 1
BsaWI TCCGGT 1
BsaXI NNNNNNNNNNNNNACNNNNNCTCCNNNNNNNNNNN 6
BseRI GAGGAGNNNNNNNNNNN 16
BseYI CCCAGC 1
BsgI GTGCAGNNNNNNNNNNNNNNNNN 22
BsiEI CGACCG 4
BsiEI CGATCG 4
BsiEI CGGCCG 4
BsiEI CGGTCG 4
BsiHKAI GAGCAC 5
BsiHKAI GAGCTC 5
BsiHKAI GTGCAC 5
BsiHKAI GTGCTC 5
BsiWI CGTACG 1
BsmI GAATGCNN 7
BsmBI CGTCTCNNNNNN 7
BsoBI CCCGAG 1
BsoBI CCCGGG 1
BsoBI CTCGAG 1
BsoBI CTCGGG 1
Bsp1286I GDGCHC 5
BspDI ATCGAT 2
BspEI TCCGGA 1
BspHI TCATGA 1
BspMI ACCTGCNNNNNNNNN 10
BspQI GCTCTTCN 8
BsrBI CCGCTC 3
BsrDI GCAATGNNN 8
BsrFI ACCGGC 1
BsrFI ACCGGT 1
BsrFI GCCGGC 1
BsrFI GCCGGT 1
BsrGI TGTACA 1
BssHII GCGCGC 1
BstAPI GCANNNNNTGC 7
BstBI TTCGAA 2
BstEII GGTNACC 1
BstXI CCANNNNNNTGG 8
BstYI AGATCC 1
BstYI AGATCT 1
BstYI GGATCC 1
BstYI GGATCT 1
BstZ17I GTATAC 3
Bsu36I CCTNAGG 2
BtgI CCACGG 1
BtgI CCATGG 1
BtgI CCGCGG 1
BtgI CCGTGG 1
BtgZI GCGATGNNNNNNNNNNNNNNN 16
ClaI ATCGAT 2
DraI TTTAAA 3
DraIII CACNNNGTG 6
DrdI GACNNNNNNGTC 7
EaeI CGGCCA 1
EaeI CGGCCG 1
EaeI TGGCCA 1
EaeI TGGCCG 1
EagI CGGCCG 1
EarI CTCTTCNNNNN 7
Eco57MI CTGAAGNNNNNNNNNNNNNNNNN 22
Eco57MI CTGGAGNNNNNNNNNNNNNNNNN 22
EcoNI CCTNNNNNAGG 5
EcoO109I AGGNCCC 2
EcoO109I AGGNCCT 2
EcoO109I GGGNCCC 2
EcoO109I GGGNCCT 2
EcoP15I CAGCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNN 31
EcoRI GAATTC 1
EcoRV GATATC 3
FalI NNNNNNNNNNNNNNAAGNNNNNCTTNNNNNNNNNNNNNN 6
FseI GGCCGGCC 6
FspI TGCGCA 3
FspAI ATGCGCAC 4
FspAI ATGCGCAT 4
FspAI GTGCGCAC 4
FspAI GTGCGCAT 4
HaeII AGCGCC 5
HaeII AGCGCT 5
HaeII GGCGCC 5
HaeII GGCGCT 5
HincII GTCAAC 3
HincII GTCGAC 3
HincII GTTAAC 3
HincII GTTGAC 3
HindIII AAGCTT 1
KasI GGCGCC 1
KpnI GGTACC 5
MfeI CAATTG 1
MluI ACGCGT 1
MmeI TCCAACNNNNNNNNNNNNNNNNNNNNN 26
MmeI TCCGACNNNNNNNNNNNNNNNNNNNNN 26
MscI TGGCCA 3
MslI CACNNNNATG 5
MslI CACNNNNGTG 5
MslI CATNNNNATG 5
MslI CATNNNNGTG 5
MspA1I CAGCGG 3
MspA1I CAGCTG 3
MspA1I CCGCGG 3
MspA1I CCGCTG 3
NaeI GCCGGC 3
NarI GGCGCC 2
NcoI CCATGG 1
NdeI CATATG 2
NgoMIV GCCGGC 1
NheI GCTAGC 1
NotI GCGGCCGC 2
NruI TCGCGA 3
NsiI ATGCAT 5
NspI ACATGC 5
NspI ACATGT 5
NspI GCATGC 5
NspI GCATGT 5
PacI TTAATTAA 5
PaeR7I CTCGAG 1
PasI CCCAGGG 2
PasI CCCTGGG 2
PciI ACATGT 1
PflFI GACNNNGTC 4
PflMI CCANNNNNTGG 7
PfoI TCCNGGA 1
PmeI GTTTAAAC 4
PmlI CACGTG 3
PpuMI AGGWCCC 2
PpuMI AGGWCCT 2
PpuMI GGGWCCC 2
PpuMI GGGWCCT 2
PshAI GACNNNNGTC 5
PsiI TTATAA 3
PspOMI GGGCCC 1
PspXI CTCGAGB 2
PstI CTGCAG 5 
PvuI CGATCG 4
PvuII CAGCTG 3
RsrII CGGACCG 2
RsrII CGGTCCG 2
SacI GAGCTC 5
SacII CCGCGG 4
SalI GTCGAC 1
SapI GCTCTTCNNNNN 8
SbfI CCTGCAGG 6
ScaI AGTACT 3
SexAI ACCAGGT 1
SexAI ACCTGGT 1
SfcI CTACAG 1
SfcI CTATAG 1
SfcI CTGCAG 1
SfcI CTGTAG 1
SfiI GGCCNNNNNGGCC 8
SfoI GGCGCC 3
SgrAI CACCGGCG 2
SgrAI CACCGGTG 2
SgrAI CGCCGGCG 2
SgrAI CGCCGGTG 2
SmaI CCCGGG 3
SmlI CTCAAG 1
SmlI CTCGAG 1
SmlI CTTAAG 1
SmlI CTTGAG 1
SnaBI TACGTA 3
SpeI ACTAGT 1
SspI AATATT 3
StuI AGGCCT 3
StyI CCAAGG 1
StyI CCATGG 1
StyI CCTAGG 1
StyI CCTTGG 1
SwaI ATTTAAAT 4
TatI AGTACA 1
TatI AGTACT 1
TatI TGTACA 1
TatI TGTACT 1
Tth111I GACNNNGTC 4
XbaI TCTAGA 1
XcmI CCANNNNNNNNNTGG 8
XhoI CTCGAG 1
XmaI CCCGGG 1
XmnI GAANNNNTTC 5
ZraI GACGTC 3
