Origami is the traditional Japanese folk art of paper folding. The goal of this art is to transform a flat sheet of material into a finished sculpture through folding and sculpting techniques.

DNA origami is the nanoscale folding of DNA to create arbitrary two and three dimensional shapes at the nanoscale. The specificity of the interactions between complementary base pairs makes DNA a useful construction material through design of its base sequences.

DNA origami is made from long strands of viral DNA. To fold it up, small pieces of 'helper' DNA are used - which are pre-coded to interact, (via Watson-Crick base pairing), at specific points on the strand. These can be configured to weave the DNA strand back and forth, generating a pattern.

We have illustrated our workflow for the 2D Gujarat Map below.

Figure 1 | A basic structure of the Map of Gujarat on caDNAno.

Figure 2 | Gujarat Map with scaffold crossovers installed.

Figure 3 | Gujarat Map with staplers installed and corrected.

Figure 4 | Gujarat Map with staplers colored regionwise.

Figure 5 | The kinds of stapler crossovers that we used in the structure.